fitc filters mammalian cell cryopreservation container  (Thermo Fisher)

Journal: Frontiers in Cellular Neuroscience

Article Title: Cryopreservation of primary neonatal rat oligodendrocytes and recapitulation of in vitro oligodendrocyte characteristics

doi: 10.3389/fncel.2024.1520992

Figure Lengend Snippet: Viability and OL lineage purity of cryopreserved OPCs. (A) Illustration of the pipeline for primary OL culture and cryopreservation. (B) Representative images of control and cryopreserved OPCs after 2 days of stabilization. Immunostaining for NG2 and Olig2. (C) Graph of viability measured by a dual-fluorescence cell counter utilizing AO/PI ( n = 4 biologically independent experiments per group, with three experimental groups. Statistical analysis was performed using a two-sided Kruskal–Wallis test. Data are presented as mean ± SEM). (D) Graph of OL lineage purity measured as Olig2 + DAPI + OPCs/DAPI + total cells (%) ( n = 4 biologically independent experiments per group, with three experimental groups. For each group, 10 images were analyzed, with approximately 75 DAPI + cells per image. Statistical analysis was performed using a two-sided Kruskal–Wallis test. Data are presented as mean ± SEM). OPC, oligodendrocyte progenitor cell; PDGF, platelet-derived growth factor; FGF, fibroblast growth factor; EGF, epidermal growth factor; T3, thyroid hormone T3; AO/PI, acridine orange/propidium iodide.

Article Snippet: The cell suspension in CELLBANKER 2 was transferred to cryopreservation vials (Thermo Fisher Scientific, #368632), two vials/flask, 1 mL/vial, and the vials were placed in a cell cryopreservation container (Thermo Fisher Scientific, #5100-0001) utilizing isopropanol.

Techniques: Control, Immunostaining, Fluorescence, Derivative Assay